黄斑篮子鱼去毒相关基因的克隆与肝脏组成型表达分析   总被引：1，自引：0，他引：1  

王琳  梁旭方  林群  李光照  胡永乐 《热带海洋学报》2009,28(6):79-87

从基因水平探讨海洋鱼类对海洋藻毒素的去毒分子机理。采用RT-PCR法成功克隆了黄斑篮子鱼Siganus oramin肝脏I时相代谢酶细胞色素P450 1A(CYP1A)、II时相代谢酶alpha型谷胱甘肽S-转移酶(GSTA)和rho型谷胱甘肽S-转移酶(GSTR)、热休克蛋白70 (HSP70)、alpha 1型钠钾ATP酶(ATP1A1)及β-肌动蛋白(beta-actin, ACT)基因cDNA核心序列，序列分别长879 bp、582 bp、588 bp、660 bp、749 bp和554 bp。序列同源性分析发现，属鲈形目的黄斑篮子鱼CYP1A、GSTA和GSTR与同属鲈形目的牙鲆Paralichthys olivaceus、欧洲鲽Pleuronectes platessa、真鲷Pagrus major、鲤形目的斑马鱼Brachydanio rerio 相应氨基酸序列同源性较高，CYP1A和GSTA与非洲爪蟾(两栖类)、鸡(鸟类)、小鼠、大鼠和人(哺乳类)相应氨基酸序列同源性低，这可能与鱼类I、II时相去毒酶基因承担水环境毒素去毒代谢的特殊功能有关；而HSP70、ATP1A和β-肌动蛋白在鱼类、两栖类、鸟类、哺乳类中均有较高的同源性，这可能与这些基因在机体中承担的最基本的生命功能相关。应用半定量RT-PCR的方法，以β-肌动蛋白作为外参照，在指数期增长范围内分别得到了CYP1A、GSTA、GSTR、HSP70和ATP1A1 mRNA与β-肌动蛋白mRNA (%)的比值，确定黄斑篮子鱼肝脏去毒相关基因的组成型表达水平。其中，黄斑篮子鱼肝脏CYP1A、GSTA和GSTR基因组成型表达相对较高，HSP70和ATP1A1基因组成型表达相对较低，这可能与不同基因在黄斑篮子鱼海洋藻毒素去毒分子机理中承担的作用相关，为海洋藻毒素在海洋鱼类中的积聚及代谢去毒分子机制的研究提供了相关数据。  相似文献   

雄性半滑舌鳎雄激素受体基因的克隆与表达分析     

温海深  张葭人  陈彩芳  金国雄  陈晓燕  史宝  何峰 《中国海洋大学学报(自然科学版)》2009,39(3)

利用RT-PCR方法从半滑舌鳎(Cynoglossus semilaevis)中克隆了雄激素受体(Androgen receptor,AR)基因cDNA的部分序列(434 bp),经BLAST比对与狼鲈(Dicentrarchus labrax)、花溪(Kryptolebias marmoratus)、三斑海猪鱼(Halichoeres trimaculatus)和三刺鱼(Gasterosteus aculeatus)的雄激素受体同源性分别为84%,82%,82%和81%.组织表达分析表明,AR基因在半滑舌鳎的性腺、肝、胃、脾、肾、头肾、肠、鳃、心、脑和肌肉这11种组织中均有表达,表达量有所差异.肾中表达量最丰富,鳃中最少.根据其他硬骨鱼类表达模式,推测半滑舌鳎可能只含有1种AR.  相似文献   

CSAMT勘探中场区、记录规则、阴影及场源复印效应的解析研究   总被引：22，自引：9，他引：13       下载免费PDF全文

陈明生  闫述 《地球物理学报》2005,48(4):951-958

由矢量位导出均匀大地表面上水平接地谐变电偶极子地下电磁场的闭合表达式, 将地层波和地面波显式地区分开来, 据此定义了与远区场和近区场对应的地面波区和地层波区. 对长期困扰CSAMT勘探工作者的记录点问题、阴影和场源复印效应问题给出了物理解释. 由地层波与地面波之比给出了定量划分场区的方法, 每一场分量最佳记录规则下的条件和判断是产生阴影和场源复印效应的前提. 山西沁水盆地CSAMT勘探覆盖点的推断结果表明, 阴影和场源复印效应是由地层波所携带的源与观测点之间、源下方真实地质情况的信息决定, 是可以利用的.  相似文献   

The second order characteristic condition for plastic flow     

Graham Weir  Roger Young 《国际地质力学数值与分析法杂志》2003,27(15):1299-1316

In this paper the second order characteristic (discontinuous bifurcation) condition is derived for the granular flow (fully plastic) equations. This second order bifurcation equation is shown to be formally identical to the first order localization requirement during steady elastoplastic deformation provided the elastic compliance tensor is substituted for the product of the plastic multiplier with the flow Hessian. For isotropic yield and flow functions the invariant form of the characteristic condition is given in detail, as well as an alternative expression in adapted co‐ordinates. The characteristic condition can be regarded as defining a hardening function which is maximized to identify the critical angles. When the method is applied to 3D Coulomb flow, Mohr's 3D fracture plane conditions are obtained uniquely. Copyright © 2003 John Wiley & Sons, Ltd.  相似文献   

AN ACCURATE MODEL FOR CALCULATING CORRECTION OF PATH FLEXURE OF SATELLITE SIGNALS     

Li Yanxing  Hu Xinkang  Shuai Ping  Zhang Zhongfu 《大地测量与地球动力学》2003,23(Z1)

1　IntroductionTherefractionoflight electronicsignalpropa gatinginatmospherehasbeenstudiedbymanysci entistsandsomemodelofatmosphericrefractioncorreetionhaveheenestablished ,includingHopfieldSaastamoinen,DavisandMarinimodels[1 ] .Butthesemodelscanbeusedonlytocalculatethecorrec tionfortherefractionduetoatmosphericdensityvariation ,whilethecorrectionfortherefractionduetothesignalpathflexurecannotbecalculated .Hop fieldhasestimatedsignalpathflexureerrorbythemethodofraytracing[2 ] ,butastrictcorr…  相似文献   

Smeared crack approach: back to the original track     

M. Cervera  M. Chiumenti 《国际地质力学数值与分析法杂志》2006,30(12):1173-1199

This paper briefly reviews the formulations used over the last 40 years for the solution of problems involving tensile cracking, with both the discrete and the smeared crack approaches. The paper focuses on the smeared approach, identifying as its main drawbacks the observed mesh‐size and mesh‐bias spurious dependence when the method is applied ‘straightly’. A simple isotropic local damage constitutive model is considered, and the (exponential) softening modulus is regularized according to the material fracture energy and the element size. The continuum and discrete mechanical problems corresponding to both the weak discontinuity (smeared cracks) and the strong discontinuity (discrete cracks) approaches are analysed and the question of propagation of the strain localization band (crack) is identified as the main difficulty to be overcome in the numerical procedure. A tracking technique is used to ensure stability of the solution, attaining the necessary convergence properties of the corresponding discrete finite element formulation. Numerical examples show that the formulation derived is stable and remarkably robust. As a consequence, the results obtained do not suffer from spurious mesh‐size or mesh‐bias dependence, comparing very favourably with those obtained with other fracture and continuum mechanics approaches. Copyright © 2006 John Wiley & Sons, Ltd.  相似文献   

Strain localization in sand: an overview of the experimental results obtained in Grenoble using stereophotogrammetry     

Jacques Desrues  Gioacchino Viggiani 《国际地质力学数值与分析法杂志》2004,28(4):279-321

Experimental results are presented from the extensive program of drained plane strain compression tests on sand carried out in Grenoble over the last two decades. Systematic analysis of photographs of the deforming specimen allowed for measuring deformations and determining strain fields throughout the test, that is: prior to, at, and after the onset of strain localization. The principles, details and accuracy of the procedure are described, as well as its suitability to properly depict the patterns of deformation. Findings concerning the occurrence and progression of strain localization are discussed. The issues of shear band orientation and thickness are addressed, as well as temporary and persistent complex localization patterns, and the volumetric behaviour inside a band after its formation. The influence of such variables as initial state of the sand (effective stress and relative density), specimen size and slenderness, as well as grain size, is discussed. Copyright © 2004 John Wiley & Sons, Ltd  相似文献   

Sex expression and growth rates in natural populations of the desert soil crustal mossSyntrichia caninervis     

Lloyd R. Stark  Brent D. Mishler  D.Nicholas McLetchie 《Journal of Arid Environments》1998,40(4):401-416

The low elevation Mojave Desert cryptobiotic crust is dominated by the mossSyntrichia caninervis. In 16 populations of this moss, stem height and population were significantly associated with sex expression, with longer stems expressing sex more frequently and producing more perichaetia. The above-ground age of stems ranged up to 10 years, with a mean growth rate across populations of 0.36 mm year⁻¹. The overall stem sex ratio, as a proportion of the total number of stems, was 0.30♀: 0♂: 0.70 non-expressing. The probability of expressing sex in a given season was 0.09. The absence of sexual reproduction (no sporophytes) and likely absence of the male sex from the area indicate that the non-expressing plants are female individuals. The slow growth rates, low rates of sex expression, absence of male plants, and absence of sexual reproduction in this crustal species may help explain why re-establishment of mosses on desert soils can take decades.  相似文献   

Strain localization due to structural in-homogeneities in the Central European Basin System     

Mauro Cacace  Ulf Bayer  Anna Maria Marotta 《International Journal of Earth Sciences》2008,97(5):899-913

The large-scale crustal deformations observed in the Central European Basin System (CEBS) are the result of the interplay between several controlling factors, among which lateral rheological heterogeneities play a key role. We present a finite-element integral thin sheet model of stress and strain distribution within the CEBS. Unlike many previous models, this study is based on thermo-mechanical data to quantify the impact of lateral contrasts on the tectonic deformation. Elasto-plastic material behaviour is used for both the mantle and the crust, and the effects of the sedimentary fill are also investigated. The consistency of model results is ensured through comparisons with observed data. The results resemble the present-day dynamics and kinematics when: (1) a weak granite-like lower crust below the Elbe Fault System is modelled in contrast to a stronger lower crust in the area extending north of the Elbe Line throughout the Baltic region; and (2) a transition domain in the upper mantle is considered between the shallow mantle of the Variscan domain and the deep mantle beneath the East European Craton (EEC), extending from the Elbe Line in the south till the Tornquist Zone. The strain localizations observed along these structural contrasts strongly enhance the dominant role played by large structural domains in stiffening the propagation of tectonic deformation and in controlling the basin formation and the evolution in the CEBS.  相似文献   

中国南北过渡带范围的地理表达及定量探测     

李亚男  刘钢军  刘德新  秦耀辰 《地理研究》2021,40(7):1857-1869

气候变化下中国南北过渡带的动态变化及地域范围探测是识别农业生产敏感区、研究农业适应行为的基础。前人基于“自上而下”或“自下而上”的方法,采用不同的划界指标对中国南北过渡带的范围进行了探索,但对气候变化下南北过渡带范围的地理表达及其地域范围的定量探测较少涉及。采用1951—2018年2400多个国家气象站点气温和降水的逐日观测数据,以800 mm等降水量线、1月0℃均温、日均温≥10℃积温、日均温≥10℃日数和干燥度指数0.5为划界指标,运用ArcGIS栅格计算和均值-标准差对中国南北过渡带进行地理表达。结果表明,中国的南北分界是一条宽窄不一的过渡带,各划界指标的等值线在气候变化下变动明显,各气象要素的大致变动范围西南段较东北段更为稳定,日均温≥10℃积温和干燥度指数的变化幅度大于800 mm等降水量线和1月0℃均温。确定的中国南北过渡带的极端最北界自西向东依次穿过礼县、耀县、韩城、安泽、涉县、静海县;极端最南界自西向东依次穿过北川、宁强、西乡、房县、淅川、罗山、商城、定远、临安县。该范围内提取的637个县域中,位于南北过渡带气候变化稳定区的县域256个,位于气候变化敏感区的县域187个。研究可为中国南北过渡带农业生产适应气候变化提供科学依据。  相似文献